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The light generated from free pyrophosphate was transmitted through the base of the optical picotiter plate and detected by a large-format CCD. JPEG image file containing the pathways mapping of phenylpropanoid biosynthesis from differentially up-regulated genes in Vagad in drought condition. Nardini A, Salleo S: Limitation of stomatal conductance by hydraulic traits: sensing or preventing xylem cavitation?. These differentially up-regulated genes were further annotated through the SEA Singular Enrichment Analysis method and identified enriched gene ontology GO terms. The steady-state decline under the irrigated condition in both the accessions exhibited a similar slope; however, as drought progressed, the slope in Vagad remained the same under moderate drought and then decreased under severe drought, whereas in RAHS, the slope remained constant under irrigated and drought conditions.

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    Kutch Vagad Khabar Patrika is the daily newspaper of the prominent Kutchi Community owned and managed by the Vagad Visha Oswal Chovisi Mahajan.
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    Vagad and RAHS seedlings were grown during the summer of under field conditions at the Mahatma Gandhi Mission, Aurangabad, at Padegaon, India 19[degrees] 15' N; 75[degrees] 23' E, m above sea levelin the rainout experimental station.

    The average length of the large contigs was bp. Proc Natl Acad Sci99 11 : At these criteria, 21, genes were annotated Additional file

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    Molecular studies on cotton in response to drought are scanty.

    Plant Physiol4 : To assign the orientation of the transcripts, all the pooled exemplar sequences were analyzed by the ESTScan Model, which is trained on Arabidopsis and Oryza models.


    Their precise role in the abiotic stress response regulatory network is not fully understood [40]. Missing values were filtered, normalized, and natural log2 transformed for biological replicates. The reaction mixture was kept at 94[degrees]C for 2 min, followed by 94[degrees]C for 5 sec, 52[degrees]C for 5 sec, and 72[degrees]C for 5 sec for 40 cycles. The adaptive mechanisms in response to drought in plants are reduced water loss, reduced radiation absorption, reduced evaporation surfaces, and low tissue water potential.

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    Picture and water in VAV is visible in picture attached (). Waters in the region was [email protected], [email protected]
    Accessed 27 Nov. NP helped in drafting and revising the manuscript.

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    It was observed that Vagad and RAHS showed substantial differences in several physiological parameters and relative gene expression in response to drought. The Plant Cell7 7 : CRFs function redundantly to regulate the various metabolic functions, including transpiration, stomatal conductance, and respiration [37, 38].

    The droplets act as separate microreactors in which parallel DNA amplifications are performed while yielding approximately 10 7 copies of a template per bead.

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    Figure 3: Effect of moderate [white circle] and severe drought o on leaf dehydration curve in comparison to irrigated plants [black down-pointg triangle] of A Vagad and B RAHS accessions.

    In DT2 experiments, the cotyledonary leaves of Vagad seedlings remained green and turgid after seven days of water stress Figure 1awhereas RAHS seedlings turned pale and exhibited a drooping effect Figure 1b. Dark respiration R was measured under similar microclimatic conditions after dark adaptation of the leaf for more than 30 min. The phenyl propanoid pathways leading to coumarin and similarly, a lignin biosynthesis flavonoid pathway leading to polyketide biosynthesis such as stilbene biosynthesis were enriched during the irrigated condition in Vagad Figure 4.

    Similarly, and genes were differentially up-regulated in Vagad and RAHS, respectively, under the drought condition Additional files 4 and 5.

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