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The light generated from free pyrophosphate was transmitted through the base of the optical picotiter plate and detected by a large-format CCD. JPEG image file containing the pathways mapping of phenylpropanoid biosynthesis from differentially up-regulated genes in Vagad in drought condition. Nardini A, Salleo S: Limitation of stomatal conductance by hydraulic traits: sensing or preventing xylem cavitation?. These differentially up-regulated genes were further annotated through the SEA Singular Enrichment Analysis method and identified enriched gene ontology GO terms. The steady-state decline under the irrigated condition in both the accessions exhibited a similar slope; however, as drought progressed, the slope in Vagad remained the same under moderate drought and then decreased under severe drought, whereas in RAHS, the slope remained constant under irrigated and drought conditions.

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    Vagad and RAHS seedlings were grown during the summer of under field conditions at the Mahatma Gandhi Mission, Aurangabad, at Padegaon, India 19[degrees] 15' N; 75[degrees] 23' E, m above sea levelin the rainout experimental station.

    The average length of the large contigs was bp. Proc Natl Acad Sci99 11 : At these criteria, 21, genes were annotated Additional file

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    Molecular studies on cotton in response to drought are scanty.

    Plant Physiol4 : To assign the orientation of the transcripts, all the pooled exemplar sequences were analyzed by the ESTScan Model, which is trained on Arabidopsis and Oryza models.

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    Their precise role in the abiotic stress response regulatory network is not fully understood [40]. Missing values were filtered, normalized, and natural log2 transformed for biological replicates. The reaction mixture was kept at 94[degrees]C for 2 min, followed by 94[degrees]C for 5 sec, 52[degrees]C for 5 sec, and 72[degrees]C for 5 sec for 40 cycles. The adaptive mechanisms in response to drought in plants are reduced water loss, reduced radiation absorption, reduced evaporation surfaces, and low tissue water potential.

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    Picture and water in VAV is visible in picture attached (). Waters in the region was [email protected], [email protected]
    Accessed 27 Nov. NP helped in drafting and revising the manuscript.

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    It was observed that Vagad and RAHS showed substantial differences in several physiological parameters and relative gene expression in response to drought. The Plant Cell7 7 : CRFs function redundantly to regulate the various metabolic functions, including transpiration, stomatal conductance, and respiration [37, 38].

    The droplets act as separate microreactors in which parallel DNA amplifications are performed while yielding approximately 10 7 copies of a template per bead.

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    Figure 3: Effect of moderate [white circle] and severe drought o on leaf dehydration curve in comparison to irrigated plants [black down-pointg triangle] of A Vagad and B RAHS accessions.

    In DT2 experiments, the cotyledonary leaves of Vagad seedlings remained green and turgid after seven days of water stress Figure 1awhereas RAHS seedlings turned pale and exhibited a drooping effect Figure 1b. Dark respiration R was measured under similar microclimatic conditions after dark adaptation of the leaf for more than 30 min. The phenyl propanoid pathways leading to coumarin and similarly, a lignin biosynthesis flavonoid pathway leading to polyketide biosynthesis such as stilbene biosynthesis were enriched during the irrigated condition in Vagad Figure 4.

    Similarly, and genes were differentially up-regulated in Vagad and RAHS, respectively, under the drought condition Additional files 4 and 5.

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